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CNN3 Polyclonal Antibody

RD75417A
Reddot Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCNN3
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    CNN3 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1266
  • Target name
    CNN3
  • Target description
    calponin 3
  • Target synonyms
    calponin 3, acidic; calponin-3; dJ639P13.2.2 (acidic calponin 3)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 36 kDa. The expected band size for Calponin 3/CNN3 is at 36 kDa.
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