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ProductsBack/COL4A1 / Collagen IV Alpha1 Antibody (clone 6F6)

COL4A1 / Collagen IV Alpha1 Antibody (clone 6F6)

LS-C765700
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL4A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    COL4A1 / Collagen IV Alpha1 Antibody (clone 6F6)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC-P Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6F6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1282
  • Target name
    COL4A1
  • Target description
    collagen type IV alpha 1 chain
  • Target synonyms
    BSVD, BSVD1, COL4A1s, PADMAL, RATOR, collagen alpha-1(IV) chain, COL4A1 NC1 domain, arresten, collagen IV, alpha-1 polypeptide, collagen of basement membrane, alpha-1 chain
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.
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