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Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei

Collagen IV Polyclonal Antibody

BS-4595R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL4A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Collagen IV Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1282
  • Target name
    COL4A1
  • Target description
    collagen type IV alpha 1 chain
  • Target synonyms
    BSVD, BSVD1, COL4A1s, PADMAL, RATOR, collagen alpha-1(IV) chain, COL4A1 NC1 domain, arresten, collagen IV, alpha-1 polypeptide, collagen of basement membrane, alpha-1 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02462
  • Protein Name
    Collagen alpha-1(IV) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Song G, Luo T, Dong L, et al. Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression. Braz J Med Biol Res. 2017,50(7):e5403. doi: 10.1590/1414-431X20175403
    Read this paper
  • Bao J, Wu Q, Wang Y, et al. Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. Int J Mol Med. 2016,38(2):457-65. doi: 10.3892/ijmm.2016.2638
    Read this paper
  • Wu L, Wang Q, Guo F, et al. MicroRNA-27a Induces Mesangial Cell Injury by Targeting of PPARγ, and its In Vivo Knockdown Prevents Progression of Diabetic Nephropathy. Sci Rep. 2016,6:26072. doi: 10.1038/srep26072
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.
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