Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody (GTX130215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![COL4A1 antibody detects COL4A1 protein at vesicle by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL4A1 stained by COL4A1 antibody (GTX130215) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX130215/GTX130215_44405_20220415_ICC_IF_w_23060523_274.webp "COL4A1 antibody detects COL4A1 protein at vesicle by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL4A1 stained by COL4A1 antibody (GTX130215) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody (GTX130215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
COL4A1 antibody
GTX130215
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOL4A1
Overview
- SupplierGeneTex
- Product NameCOL4A1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.32 mg/ml
- ConjugateUnconjugated
- Gene ID1282
- Target nameCOL4A1
- Target descriptioncollagen type IV alpha 1 chain
- Target synonymsBSVD, BSVD1, COL4A1s, PADMAL, RATOR, collagen alpha-1(IV) chain, COL4A1 NC1 domain, arresten, collagen IV, alpha-1 polypeptide, collagen of basement membrane, alpha-1 chain
- HostRabbit
- IsotypeIgG
- Protein IDP02462
- Protein NameCollagen alpha-1(IV) chain
- Scientific DescriptionThis gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hui L, Nie Y, Li S, et al. Matrix metalloproteinase 9 facilitates Zika virus invasion of the testis by modulating the integrity of the blood-testis barrier. PLoS Pathog. 2020,16(4):e1008509. doi: 10.1371/journal.ppat.1008509Read this paper
Datasheet
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