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ProductsBack/Collagen II Polyclonal Antibody
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Collagen II Polyclonal Antibody

BS-0709R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCOL2A1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Collagen 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1280
  • Target name
    COL2A1
  • Target description
    collagen type II alpha 1 chain
  • Target synonyms
    alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; COL11A3; collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; SEDC; STL1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02458
  • Protein Name
    Collagen alpha-1(II) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Establishment and characterization of an immortalized human chondrocyte cell line. Yang J et al., 2020 May, Biotechnol Lett
    Read more
  • Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells. Zhan X, 2020 Mar, J Biomed Mater Res A
    Read more
  • LncRNA SNHG1 alleviates IL-1beta-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-kappaB signaling pathways. Lei J et al., 2019 Sep 30, Biosci Rep
    Read more
  • Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecular mechanism. Wang D et al., 2019 Sep, Biomed Pharmacother
    Read more
  • Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Zhao R et al., 2019 Mar, Bone Joint Res
    Read more
  • Storage solution containing hydrogen improves the preservation effect of osteochondral allograft. Han Y et al., 2019 Jun, Cell Tissue Bank
    Read more
  • Intra-articular injection of N-acetylglucosamine and hyaluronic acid combined with PLGA scaffolds for osteochondral repair in rabbits. Wang HC et al., 2018, PLoS One
    Read more
  • Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway. Zhou H et al., 2018 Dec 9, Biochem Biophys Res Commun
    Read more
  • Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats. Chen X et al., 2018 Dec 1, J Biomed Nanotechnol
    Read more
  • Effect of Cyclic Dynamic Compressive Loading on Chondrocytes and Adipose-Derived Stem Cells Co-Cultured in Highly Elastic Cryogel Scaffolds. Chen CH et al., 2018 Jan 26, Int J Mol Sci
    Read more

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Figure 1. Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cartilage tissue lysates, Lane 2: mouse cartilage tissue lysates, Lane 3: mouse auricular cartilage lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.
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