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Collagen Type II antibody

10R-C135A
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    Collagen Type II antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cartilage tissue lysates, Lane 2: mouse cartilage tissue lysates, Lane 3: mouse auricular cartilage lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.
Product group Antibodies
Boster Bio
Anti-Collagen II/COL2A1 Antibody Picoband(r)A00517-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCOL2A1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Collagen type II [M2139], Mouse IgG1, kappaAB02802-1.1
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
ReactivityBovine, Chicken, Human, Mouse, Rat
TargetCOL2A1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Mouse COL2A1 Antibody144-01560
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetCOL2A1
  • SizePrice
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
Collagen II Polyclonal AntibodyBS-0709R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetCOL2A1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200.
Product group Antibodies
Cusabio
COL2A1 Monoclonal AntibodyCSB-MA186977
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCOL2A1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
COL2A1 / Collagen II Alpha 1 Antibody (clone 1H1)LS-C765691
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCOL2A1
  • SizePrice
Product group Antibodies
MedChem Express
Collagen II Antibody (YA3615, Mouse)HY-P83918
TargetCOL2A1
  • SizePrice
Product group Antibodies
Biomatik
Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Polyclonal AntibodyCAU27370
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCOL2A1
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Product group Antibodies
Assay Genie
IPO4 AntibodyCAB15600
ReactivityHuman
TargetCOL2A1
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Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
Collagen II antibody [N1], N-termGTX100829
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCOL2A1
  • SizePrice