Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100829/GTX100829_40751_20170510_IHC-P_M_w_23060100_140.webp "Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100829/GTX100829_45511_20240906_WB_Fraction_24110700_852.webp "HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Collagen II antibody [N1], N-term
GTX100829
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOL2A1
Overview
- SupplierGeneTex
- Product NameCollagen II antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.16 mg/ml
- ConjugateUnconjugated
- Gene ID1280
- Target nameCOL2A1
- Target descriptioncollagen type II alpha 1 chain
- Target synonymsACG2, ANFH, ANFH1, AOM, COL11A3, EDMMD, LCPD, OSCDP, PLSDT, SEDC, SEDSTN, SEMDSTWK, SMDALG, STL1, VPED, collagen alpha-1(II) chain, alpha-1 type II collagen, arthroophthalmopathy, progressive (Stickler syndrome), cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide, collagen, type II, alpha 1
- HostRabbit
- IsotypeIgG
- Protein IDP02458
- Protein NameCollagen alpha-1(II) chain
- Scientific DescriptionThis gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chang YH, Ding DC, Wu KC. Human Umbilical Mesenchymal Stromal Cells Mixed with Hyaluronan Transplantation Decreased Cartilage Destruction in a Rabbit Osteoarthritis Model. Stem Cells Int. 2021,2021:2989054. doi: 10.1155/2021/2989054Read this paper
- Muhammad SA, Nordin N, Hussin P, et al. Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage. Cartilage. 2021,13(2_suppl):872S-884S. doi: 10.1177/1947603519876333Read this paper
- Ding DC, Chu TY, Liu HW. Dedifferentiation of human uterine polyp stem cells into embryo-like cells during inducing pluripotency. Int J Biol Sci. 2018,14(11):1586-1598. doi: 10.7150/ijbs.23401Read this paper
- Qu J, Lu D, Guo H, et al. PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage. Clin Exp Pharmacol Physiol. 2016,43(3):312-8. doi: 10.1111/1440-1681.12537Read this paper
Datasheet
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