
Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4°C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Collagen I Monoclonal Antibody
BSM-33400M

ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOL1A1
Overview
- SupplierBioss Antibodies
- Product NameCollagen I Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IF()
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7C7
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1277
- Target nameCOL1A1
- Target descriptioncollagen type I alpha 1 chain
- Target synonymsalpha-1 type I collagen; alpha1(I) procollagen; CAFYD; collagen alpha 1 chain type I; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; EDSARTH1; EDSC; OI1; OI2; OI3; OI4; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain
- HostMouse
- IsotypeIgG
- Protein IDP02452
- Protein NameCollagen alpha-1(I) chain
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203