Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4°C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4°C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Collagen I Monoclonal Antibody
BSM-33400M
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOL1A1
Overview
- SupplierBioss
- Product NameCollagen I Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IF()
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7C7
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1277
- Target nameCOL1A1
- Target descriptioncollagen type I alpha 1 chain
- Target synonymsCAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, collagen alpha-1(I) chain, alpha-1 type I collagen, alpha1(I) procollagen, collagen Col1-ColIII-1, collagen Col1-ColIII-2, collagen alpha 1 chain type I, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, pro-alpha-1 collagen type 1, type I proalpha 1, type I procollagen alpha 1 chain
- HostMouse
- IsotypeIgG
- Protein IDP02452
- Protein NameCollagen alpha-1(I) chain
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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