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ProductsBack/Connexin 32 Antibody (YA2960, Rabbit)

Connexin 32 Antibody (YA2960, Rabbit)

HY-P83215
MedChem Express
TargetGJB1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Connexin 32 Antibody (YA2960, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2705
  • Target name
    GJB1
  • Target description
    gap junction protein beta 1
  • Target synonyms
    CMTX, CMTX1, CX32, gap junction beta-1 protein, GAP junction 28 kDa liver protein, connexin-32, gap junction protein, beta 1, 32kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Connexin 32 Antibody (YA2960) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Connexin 32.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (A01050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (Catalog # A01050) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin-32/GJB1 at approximately 35 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.
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