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ProductsBack/GJB1 / CX32 / Connexin 32 Antibody

GJB1 / CX32 / Connexin 32 Antibody

LS-C496823
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGJB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GJB1 / CX32 / Connexin 32 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 32kDa, while the observed MW by Western blot was 32kDa/36kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:1000) The predicted MW is 32kDa, while the observed MW by Western blot was 32kDa/36kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2705
  • Target name
    GJB1
  • Target description
    gap junction protein beta 1
  • Target synonyms
    CMTX, CMTX1, CX32, gap junction beta-1 protein, GAP junction 28 kDa liver protein, connexin-32, gap junction protein, beta 1, 32kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (A01050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (Catalog # A01050) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin-32/GJB1 at approximately 35 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.
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