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Western Blot analysis of K562 cells using JAB1 Polyclonal Antibody
Western Blot analysis of K562 cells using JAB1 Polyclonal Antibody
Western Blot analysis of K562 cells using JAB1 Polyclonal Antibody

COPS5 Antibody

CSB-PA003075
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCOPS5
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Overview

  • Supplier
    Cusabio
  • Product Name
    COPS5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10987
  • Target name
    COPS5
  • Target description
    COP9 signalosome subunit 5
  • Target synonyms
    CSN5, JAB1, MOV-34, SGN5, COP9 signalosome complex subunit 5, 38 kDa Mov34 homolog, COP9 constitutive photomorphogenic homolog subunit 5, jun activation domain-binding protein 1, signalosome subunit 5, testis secretory sperm-binding protein Li 231m
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92905
  • Protein Name
    COP9 signalosome complex subunit 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of JAB1 using anti-JAB1 antibody (PB9490). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAB1 antigen affinity purified polyclonal antibody (Catalog # PB9490) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAB1 at approximately 38 kDa. The expected band size for JAB1 is at 38 kDa.
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