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IHC-P analysis of human kidney tissue section using GTX02696 COX2 antibody [COX2/3320R].
IHC-P analysis of human kidney tissue section using GTX02696 COX2 antibody [COX2/3320R].
IHC-P analysis of human kidney tissue section using GTX02696 COX2 antibody [COX2/3320R].

COX2 antibody [COX2/3320R]

GTX02696
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPTGS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    COX2 antibody [COX2/3320R]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1-2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    COX2/3320R
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5743
  • Target name
    PTGS2
  • Target description
    prostaglandin-endoperoxide synthase 2
  • Target synonyms
    COX2; COX-2; cyclooxygenase 2; cyclooxygenase 2b; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; prostaglandin G/H synthase 2; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35354
  • Protein Name
    Prostaglandin G/H synthase 2
  • Scientific Description
    Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7(-LPS) whole cell lysates, Lane 3: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.
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