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Goat anti-COX2 / PTGS2

EB05286
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPTGS2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-COX2 / PTGS2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of HepG2 and NIH3T3 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5743
  • Target name
    PTGS2
  • Target description
    prostaglandin-endoperoxide synthase 2
  • Target synonyms
    COX2; COX-2; cyclooxygenase 2; cyclooxygenase 2b; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; prostaglandin G/H synthase 2; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000954.1. Purification: Antigen affinity purified. Names and symbols: PTGS2; COX2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; prostaglandin G/H synthase and cyclooxygenase; GRIPGHS; cyclooxygenase 2b
  • Reactivity Supplier
    Human, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7(-LPS) whole cell lysates, Lane 3: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.
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