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Cpn10 Antibody (YA2190, Rabbit)

HY-P82445
MedChem Express
TargetHSPE1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cpn10 Antibody (YA2190, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3336
  • Target name
    HSPE1
  • Target description
    heat shock protein family E (Hsp10) member 1
  • Target synonyms
    10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; chaperonin 10; CPN10; early-pregnancy factor; EPF; epididymis secretory sperm binding protein; GROES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; HSP10
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cpn10 Antibody (YA2190) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2190), targeting Cpn10, with a predicted molecular weight of 11 kDa (observed band size: 11 kDa). Cpn10 Antibody (YA2190) can be used for WB, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSP10 using anti-HSP10 antibody (M06192-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP10 antigen affinity purified monoclonal antibody (Catalog # M06192-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP10 at approximately 11, 13 kDa. The expected band size for HSP10 is at 11 kDa.
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