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Confocal immunofluorescence analysis of U2OS cells using CPSF6 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using CPSF6 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using CPSF6 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

CPSF6 Polyclonal Antibody

E-AB-63831
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CPSF6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is one subunit of a cleavage factor required for 3 RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3 end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPSF6 using anti-CPSF6 antibody (A04551-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPSF6 antigen affinity purified polyclonal antibody (Catalog # A04551-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 68 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa
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