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ProductsBack/Goat anti-CRTC2 (aa542-553) Antibody

Goat anti-CRTC2 (aa542-553) Antibody

ORB107649
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCRTC2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CRTC2 antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    WB: Approx 75 to 80kDa band observed in lysates of cell line HeLa (calculated MW of 73.3kDa according to NP_859066.1). Recommended concentration: 1-3microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:64000, WB: 1-3 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200186
  • Target name
    CRTC2
  • Target description
    CREB regulated transcription coactivator 2
  • Target synonyms
    CREB-regulated transcription coactivator 2; TORC2; TORC-2; transducer of regulated cAMP response element-binding protein (CREB) 2
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to CRTC2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TORC2 using anti-TORC2 antibody (A01118-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC2 antigen affinity purified polyclonal antibody (Catalog # A01118-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC2 at approximately 73KD. The expected band size for TORC2 is at 73KD.
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