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Cystatin A Antibody (YA2760, Rabbit)

HY-P83015
MedChem Express
TargetCSTA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cystatin A Antibody (YA2760, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1475
  • Target name
    CSTA
  • Target description
    cystatin A
  • Target synonyms
    AREI; cystatin A (stefin A); cystatin AS; cystatin-A; PSS4; STF1; STFA
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cystatin A Antibody (YA2760) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2760), targeting Cystatin A, with a predicted molecular weight of 11 kDa (observed band size: 11 kDa). Cystatin A Antibody (YA2760) can be used for WB, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PB9564). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody (Catalog # PB9564) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
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