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ICC/IF analysis of PFA-fixed HepG2 cells using GTX04889 CTGF antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100
ICC/IF analysis of PFA-fixed HepG2 cells using GTX04889 CTGF antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100
ICC/IF analysis of PFA-fixed HepG2 cells using GTX04889 CTGF antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100

CTGF antibody

GTX04889
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCCN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CTGF antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1490
  • Target name
    CCN2
  • Target description
    cellular communication network factor 2
  • Target synonyms
    CCN family member 2; connective tissue growth factor; CTGF; HCS24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; insulin-like growth factor-binding protein 8; NOV2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29279
  • Protein Name
    Connective tissue growth factor
  • Scientific Description
    The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTGF using anti-CTGF antibody (PB9541). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTGF antigen affinity purified polyclonal antibody (Catalog # PB9541) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTGF at approximately 38 kDa. The expected band size for CTGF is at 38 kDa.
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