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CTGF antibody

GTX124232
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CTGF antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.42 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1490
  • Target name
    CCN2
  • Target description
    cellular communication network factor 2
  • Target synonyms
    CCN family member 2; connective tissue growth factor; CTGF; HCS24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; insulin-like growth factor-binding protein 8; NOV2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29279
  • Protein Name
    Connective tissue growth factor
  • Scientific Description
    The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A novel ocular function for decorin in the aqueous humor outflow. Schneider M et al., 2021 Mar, Matrix Biol
    Read more
  • Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes. Arellano-Buendia AS et al., 2020 Nov 15, Antioxidants (Basel)
    Read more
  • Up-regulated acylglycerol kinase (AGK) expression associates with gastric cancer progression through the formation of a novel YAP1-AGK-positive loop. Huang S et al., 2020 Oct, J Cell Mol Med
    Read more
  • Blueberry Prevents the Bladder Dysfunction in Bladder Outlet Obstruction Rats by Attenuating Oxidative Stress and Suppressing Bladder Remodeling. Miyazaki N et al., 2020 May 1, Nutrients
    Read more
  • Sophoridine inhibits lung cancer cell growth and enhances cisplatin sensitivity through activation of the p53 and Hippo signaling pathways. Zhu L et al., 2020 Jun 5, Gene
    Read more
  • Bis-Indole-Derived Nuclear Receptor 4A1 (NR4A1, Nur77) Ligands as Inhibitors of Endometriosis. Mohankumar K et al., 2020 Apr 1, Endocrinology
    Read more
  • Mesenchyme-specific deletion of Tgf-beta1 in the embryonic lung disrupts branching morphogenesis and induces lung hypoplasia. Noe N et al., 2019 Sep, Lab Invest
    Read more
  • COA-Cl prevented TGF-beta1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis. Nakai K et al., 2019 Apr, J Dermatol Sci
    Read more
  • Cyclin G1 and TASCC regulate kidney epithelial cell G2-M arrest and fibrotic maladaptive repair. Canaud G et al., 2019 Jan 23, Sci Transl Med
    Read more
  • PTGFR activation promotes the expression of PTGS-2 and growth factors via activation of the PKC signaling pathway in bovine endometrial epithelial cells. Gao L et al., 2018 Dec, Anim Reprod Sci
    Read more

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Figure 1. Western blot analysis of CTGF using anti-CTGF antibody (PB9541). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTGF antigen affinity purified polyclonal antibody (Catalog # PB9541) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTGF at approximately 38 kDa. The expected band size for CTGF is at 38 kDa.
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