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ProductsBack/Cullin 4a antibody [GT885]
Cullin 4a antibody [GT885] detects Cullin 4a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4a protein stained by Cullin 4a antibody [GT885] (GTX634164) diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Cullin 4a antibody [GT885] detects Cullin 4a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4a protein stained by Cullin 4a antibody [GT885] (GTX634164) diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Cullin 4a antibody [GT885] detects Cullin 4a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cullin 4a protein stained by Cullin 4a antibody [GT885] (GTX634164) diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.

Cullin 4a antibody [GT885]

GTX634164
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCUL4A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cullin 4a antibody [GT885]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT885
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8451
  • Target name
    CUL4A
  • Target description
    cullin 4A
  • Target synonyms
    cullin-4A, CUL-4A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13619
  • Protein Name
    Cullin-4A
  • Scientific Description
    CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lai TC, Hu MC. Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism. Sci Rep. 2019,9(1):5304. doi: 10.1038/s41598-019-41411-x
    Read this paper

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Figure 1. Western blot analysis of Cullin 4a using anti-Cullin 4a antibody (A01579-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4a antigen affinity purified polyclonal antibody (Catalog # A01579-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4a at approximately 88KD. The expected band size for Cullin 4a is at 88KD.
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