Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:500.
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629000/GTX629000_44503_20211112_WB_w_23061202_289.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [GT1066] (GTX629000). Western blot analysis was performed using ADAR1 antibody [GT1066] (GTX629000). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629000/GTX629000_41288_20150612_IP_w_23061202_753.webp "Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [GT1066] (GTX629000). Western blot analysis was performed using ADAR1 antibody [GT1066] (GTX629000). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: ADAR1 protein stained by ADAR1 antibody [GT1066] (GTX629000) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629000/GTX629000_41288_IFA_w_23061202_642.webp "ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: ADAR1 protein stained by ADAR1 antibody [GT1066] (GTX629000) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:500.
ADAR1 antibody [GT1066]
GTX629000
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetADAR
Overview
- SupplierGeneTex
- Product NameADAR1 antibody [GT1066]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1066
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID103
- Target nameADAR
- Target descriptionadenosine deaminase RNA specific
- Target synonymsADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, double-stranded RNA-specific adenosine deaminase, 136 kDa double-stranded RNA-binding protein, adenosine deaminase acting on RNA 1-A, dsRNA adenosine deaminase, dsRNA adeonosine deaminase, interferon-induced protein 4, interferon-inducible protein 4
- HostMouse
- IsotypeIgG1
- Protein IDP55265
- Protein NameDouble-stranded RNA-specific adenosine deaminase
- Scientific DescriptionThis gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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