Figure 1. Western blot analysis of Aromatase using anti-Aromatase antibody (A00071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.
Figure 1. Western blot analysis of Aromatase using anti-Aromatase antibody (A00071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.
Anti-Aromatase/CYP19A1 Antibody Picoband(r)
A00071
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYP19A1
Overview
- SupplierBoster Bio
- Product NameAnti-Aromatase Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- Applications SupplierFC, IF, IHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID1588
- Target nameCYP19A1
- Target descriptioncytochrome P450 family 19 subfamily A member 1
- Target synonymsARO; ARO1; aromatase; CPV1; CYAR; CYP19; CYPXIX; cytochrome P450 19A1; cytochrome P450, family 19, subfamily A, polypeptide 1; cytochrome P450, subfamily XIX (aromatization of androgens); cytochrome P-450AROM; estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; microsomal monooxygenase; P-450AROM
- HostRabbit
- IsotypeIgG
- Protein IDP11511
- Protein NameAromatase
- Scientific DescriptionBoster Bio Anti-Aromatase/CYP19A1 Antibody Picoband® catalog # A00071. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- ETA-mediated anti-TNF-alpha therapy ameliorates the phenotype of PCOS model induced by letrozole. Lang Q et al., 2019, PLoS OneRead more