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ProductsBack/Goat anti-TRAP2 / Proteasome subunit 26S

Goat anti-TRAP2 / Proteasome subunit 26S

EB06572
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPSMD2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TRAP2 / Proteasome subunit 26S Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5708
  • Target name
    PSMD2
  • Target description
    proteasome 26S subunit ubiquitin receptor, non-ATPase 2
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 2; 55.11 protein; P97; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; proteasome 26S subunit, non-ATPase 2; protein 55.11; RPN1; S2; TNFR-associated protein 2; TRAP2; tumor necrosis factor type 1 receptor-associated protein 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002799.3; NP_001265637.1; NP_001265638.1. Purification: Antigen affinity purified. Names and symbols: PSMD2; TRAP2; MGC14274; S2; P97; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; 55.11 protein; TNFR-associated protein 2; 26S proteasome subunit p97; 26S proteasome regulatory subunit S2; 26S proteasome non-ATPase regulatory subunit 2; tumor
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD2 using anti-PSMD2 antibody (A06642-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD2 antigen affinity purified polyclonal antibody (Catalog # A06642-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD2 at approximately 97 kDa. The expected band size for PSMD2 is at 97,100 kDa.
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