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Western Blot analysis of mouse spleen cells using BLC Polyclonal Antibody
Western Blot analysis of mouse spleen cells using BLC Polyclonal Antibody
Western Blot analysis of mouse spleen cells using BLC Polyclonal Antibody

CXCL13 Antibody

CSB-PA005137
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCXCL13
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Overview

  • Supplier
    Cusabio
  • Product Name
    CXCL13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10563
  • Target name
    CXCL13
  • Target description
    C-X-C motif chemokine ligand 13
  • Target synonyms
    ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13, C-X-C motif chemokine 13, B-cell chemoattractant, B-cell-attracting chemokine 1, B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1), B-lymphocyte chemoattractant, CXC chemokine BLC, b cell-attracting chemokine 1, b lymphocyte chemoattractant, chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant), small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant), small-inducible cytokine B13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43927
  • Protein Name
    C-X-C motif chemokine 13
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (A02678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CXCL13 protein. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCA1/CXCL13 antigen affinity purified polyclonal antibody (Catalog # A02678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCA1/CXCL13 at approximately 9 kDa.
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