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Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining

CXCL13 Polyclonal Antibody

BS-2553R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCXCL13
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CXCL13 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10563
  • Target name
    CXCL13
  • Target description
    C-X-C motif chemokine ligand 13
  • Target synonyms
    ANGIE; ANGIE2; b cell-attracting chemokine 1; b lymphocyte chemoattractant; BCA1; BCA-1; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC chemokine BLC; C-X-C motif chemokine 13; SCYB13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); small-inducible cytokine B13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43927
  • Protein Name
    C-X-C motif chemokine 13
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Up-regulation of chemokine CXCL13 in systemic candidiasis. Li C et al., 2018 Jun, Clin Immunol
    Read more

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Figure 1. Western blot analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (A02678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CXCL13 protein. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCA1/CXCL13 antigen affinity purified polyclonal antibody (Catalog # A02678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCA1/CXCL13 at approximately 9 kDa.
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