Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Boiled and unboiled rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250117_WB_R_spleen_ub_25012200_955.webp "Boiled and unboiled rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250117_WB_M_tissue_ub_25012200_593.webp "Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Untreated (–) and treated (+) unboiled Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250221_WB_treatment_PNGaseF_25022521_795.webp "Untreated (–) and treated (+) unboiled Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250418_IHC-P_Multiple_RPKM_25050623_812.webp "CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250418_IHC-P_M_Multiple_RPKM_25050623_911.webp "CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250418_IHC-P_25050623_606.webp "CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_T-45656_20250515_WB_multiple_B_25052202_939.webp "Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse colon. Green: CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250. Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641880/GTX641880_45726_20250704_IHC-Fr_M_25071023_635.webp "CXCR5 antibody [HL3708] detects CXCR5 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse colon. Green: CXCR5 stained by CXCR5 antibody [HL3708] (GTX641880) diluted at 1:250. Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000. Blue: Hoechst 33342 staining.")
Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3708] (GTX641880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
CXCR5 antibody [HL3708]
GTX641880
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXCR5
Overview
- SupplierGeneTex
- Product NameCXCR5 antibody [HL3708]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3708
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID643
- Target nameCXCR5
- Target descriptionC-X-C motif chemokine receptor 5
- Target synonymsBLR1, CD185, MDR15, C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5), Burkitt lymphoma receptor 1, GTP-binding protein, CXC-R5, CXCR-5, MDR-15, chemokine (C-X-C motif) receptor 5, monocyte-derived receptor 15
- HostRabbit
- IsotypeIgG
- Protein IDP32302
- Protein NameC-X-C chemokine receptor type 5
- Scientific DescriptionThis gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitts lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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