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ProductsBack/Cyclin A2 antibody

Cyclin A2 antibody

GTX11041
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCCNA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclin A2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID890
  • Target name
    CCNA2
  • Target description
    cyclin A2
  • Target synonyms
    CCN1, CCNA, cyclin-A2, cyclin-A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20248
  • Protein Name
    Cyclin-A2
  • Scientific Description
    The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A2 antigen affinity purified polyclonal antibody (Catalog # PB9424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A2 at approximately 55 kDa. The expected band size for Cyclin A2 is at 49 kDa.
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