![Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634420/GTX634420_43024_20180525_IHC-P_H_2_w_23061202_573.webp)
Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cyclin A2 antibody [GT2547]
GTX634420
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCNA2
Overview
- SupplierGeneTex
- Product NameCyclin A2 antibody [GT2547]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT2547
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID890
- Target nameCCNA2
- Target descriptioncyclin A2
- Target synonymsCCN1, CCNA, cyclin-A2, cyclin-A
- HostMouse
- IsotypeIgG1
- Protein IDP20248
- Protein NameCyclin-A2
- Scientific DescriptionThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Larrieu D, Brunet M, Vargas C, et al. The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability. Sci Rep. 2020,10(1):789. doi: 10.1038/s41598-020-57762-9Read this paper
![Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634420/GTX634420_43024_20180525_IHC-P_H_w_23061202_249.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634420/GTX634420_43024_20171117_WB_w_23061202_219.webp)
![Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634420/GTX634420_43024_20180525_IHC-P_H_1_w_23061202_102.webp)
![Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm. Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634420/GTX634420_43024_20171220_ICC_IF_w_23061202_187.webp)



