More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Cyclin A2 antibody
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Cyclin A2 antibody

GTX103042
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCCNA2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclin A2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.73 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID890
  • Target name
    CCNA2
  • Target description
    cyclin A2
  • Target synonyms
    CCN1, CCNA, cyclin-A2, cyclin-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20248
  • Protein Name
    Cyclin-A2
  • Scientific Description
    The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of COS7 cells using Cyclin A Polyclonal Antibody
Product group Antibodies
Cusabio
CCNA1/CCNA2 AntibodyCSB-PA001857
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetCCNA2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CCNA2 Antibody144-07632
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCNA2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CCNA2 / Cyclin A2 AntibodyLS-C831473
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCCNA2
  • SizePrice
Product group Antibodies
MedChem Express
Cyclin A2 Antibody (YA487, Rabbit)HY-P80096
TargetCCNA2
  • SizePrice
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Product group Antibodies
Atlas Antibodies
Anti-CCNA2 AntibodyHPA020626
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCCNA2
  • SizePrice
Figure 1. Western blot analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A2 antigen affinity purified polyclonal antibody (Catalog # PB9424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A2 at approximately 55 kDa. The expected band size for Cyclin A2 is at 49 kDa.
Product group Antibodies
Boster Bio
Anti-Cyclin A2/CCNA2 Antibody Picoband(r)PB9424-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCNA2
  • SizePrice
Product group Antibodies
Assay Genie
CCNA1/CCNA2 AntibodyPACO06389
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetCCNA2
  • SizePrice
Product group Antibodies
GeneTex
Cyclin A2 antibodyGTX11041
ApplicationsWestern Blot
ReactivityHuman
TargetCCNA2
  • SizePrice
Cyclin A2 antibody [GT2547] detects Cyclin A2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Cyclin A2 stained by Cyclin A2 antibody [GT2547] (GTX634420) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
Cyclin A2 antibody [GT2547]GTX634420
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCCNA2
  • SizePrice
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-Cyclin A2 Polyclonal Antibody (bs-0571R), Unconjugated at 1:100, followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
CCNA2 Polyclonal AntibodyBS-0571R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCCNA2
  • SizePrice