Cyclin B1 Antibody / CCNB1

Biorbyt

Cyclin B1 Antibody / CCNB1

10

Optimal dilution of the recombinant Cyclin B1 antibody should be determined by the researcher.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 1-2ug/ml IHC-P

Research Use Only

Monoclonal

CCNB1/9242R

Unconjugated

CCNB1

cyclin B1

CCNB, G2/mitotic-specific cyclin-B1

Rabbit

IgG

G2/mitotic-specific cyclin-B1

In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephos phorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physio logical substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.

Human

-20°C,2°C to 8°C

12352203