![Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628546/GTX628546_41190_20180525_IHC-P_H_1_w_23061202_562.webp)
Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cyclin B1 antibody [GT238]
GTX628546
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCNB1
Overview
- SupplierGeneTex
- Product NameCyclin B1 antibody [GT238]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT238
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID891
- Target nameCCNB1
- Target descriptioncyclin B1
- Target synonymsCCNB, G2/mitotic-specific cyclin-B1
- HostMouse
- IsotypeIgG1
- Protein IDP14635
- Protein NameG2/mitotic-specific cyclin-B1
- Scientific DescriptionThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis. A. 30 μg U2OS whole cell lysate/extract (untreated) B. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 10 % SDS-PAGE Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:1000 Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis. A. 30 μg U2OS whole cell lysate/extract (untreated) B. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 10 % SDS-PAGE Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX628546/GTX628546_41190_WB_Nocodazole_w_23061202_475.webp)
![Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract (untreated) B. 30 μg HeLa whole cell lysate/extract (serum starvation for 48hr) C. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 3.5hr) D. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 12hr) 10 % SDS-PAGE Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:500 Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract (untreated) B. 30 μg HeLa whole cell lysate/extract (serum starvation for 48hr) C. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 3.5hr) D. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 12hr) 10 % SDS-PAGE Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX628546/GTX628546_41190_WB_double-thymidine_w_23061202_235.webp)
![Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628546/GTX628546_41190_20180525_IHC-P_H_w_23061202_454.webp)

![FACS analysis of HeLa cells using GTX82750 Cyclin B1 antibody [5G6]. Green : Cyclin B1 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX82750/GTX82750_20170912_FACS_w_23061322_196.webp)





