GeneTex anti-Cyclin B1 antibody (GTX22096) was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
GeneTex anti-Cyclin B1 antibody (GTX22096) was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Cyclin B1 antibody
GTX22096
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCNB1
Overview
- SupplierGeneTex
- Product NameCyclin B1 antibody
- Delivery Days Customer9
- Antibody SpecificityNo reaction was observed against other related cyclins.
- Application Supplier NoteWB: 1:500-1:1000. IHC-P: 1:500. IP: 1:100. ELISA: 1:2000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID891
- Target nameCCNB1
- Target descriptioncyclin B1
- Target synonymsCCNB; G2/mitotic-specific cyclin-B1
- HostRabbit
- IsotypeIgG
- Protein IDP14635
- Protein NameG2/mitotic-specific cyclin-B1
- Scientific DescriptionIn mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203