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Western Blot analysis of various samples using Cyclin B1 Polyclonal Antibody at dilution of 1:500.
Western Blot analysis of various samples using Cyclin B1 Polyclonal Antibody at dilution of 1:500.
Western Blot analysis of various samples using Cyclin B1 Polyclonal Antibody at dilution of 1:500.

Cyclin B1 Polyclonal Antibody

E-AB-70114
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    Cyclin B1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
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References
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TargetCCNB1
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Product group Antibodies
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