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ProductsBack/Cyclophilin B antibody [11C11]
FACS analysis of U2OS cells using GTX04262 Cyclophilin B antibody [11C11]. Antibody amount : 1microg/1x106 cells Blue : Primary antibody Green : Isotype control Red : Unlabelled sample
FACS analysis of U2OS cells using GTX04262 Cyclophilin B antibody [11C11]. Antibody amount : 1microg/1x106 cells Blue : Primary antibody Green : Isotype control Red : Unlabelled sample
FACS analysis of U2OS cells using GTX04262 Cyclophilin B antibody [11C11]. Antibody amount : 1microg/1x106 cells Blue : Primary antibody Green : Isotype control Red : Unlabelled sample

Cyclophilin B antibody [11C11]

GTX04262
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPIB
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclophilin B antibody [11C11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5 microg/mL. ICC/IF: 2 microg/mL. IHC-P: 0.5-1 microg/mL. FACS: 1-3 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11C11
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5479
  • Target name
    PPIB
  • Target description
    peptidylprolyl isomerase B
  • Target synonyms
    B; cyclophilin-like protein; CYPB; CYP-S1; epididymis secretory protein Li 39; HEL-S-39; OI9; peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase B; rotamase B; S-cyclophilin; SCYLP
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP23284
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase B
  • Scientific Description
    The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (A03229). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B antigen affinity purified polyclonal antibody (Catalog # A03229) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 19 kDa. The expected band size for Cyclophilin B is at 24 kDa.
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Cell lysates of HepG2(30ug) and HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
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