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Cyclophilin B Monoclonal Antibody

BSM-33228M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPIB
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclophilin B Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:1000-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C7
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5479
  • Target name
    PPIB
  • Target description
    peptidylprolyl isomerase B
  • Target synonyms
    CYP-S1, CYPB, HEL-S-39, OI9, SCYLP, peptidyl-prolyl cis-trans isomerase B, PPIase B, S-cyclophilin, cyclophilin-like protein, epididymis secretory protein Li 39, peptidylprolyl isomerase B (cyclophilin B), rotamase B
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP23284
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase B
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (A03229). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B antigen affinity purified polyclonal antibody (Catalog # A03229) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 19 kDa. The expected band size for Cyclophilin B is at 24 kDa.
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