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Western Blot analysis of K562 cells using CyPB Polyclonal Antibody.
Western Blot analysis of K562 cells using CyPB Polyclonal Antibody.
Western Blot analysis of K562 cells using CyPB Polyclonal Antibody.

PPIB Antibody

CSB-PA005769
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPIB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPIB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5479
  • Target name
    PPIB
  • Target description
    peptidylprolyl isomerase B
  • Target synonyms
    B; cyclophilin-like protein; CYPB; CYP-S1; epididymis secretory protein Li 39; HEL-S-39; OI9; peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase B; rotamase B; S-cyclophilin; SCYLP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23284
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (A03229). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B antigen affinity purified polyclonal antibody (Catalog # A03229) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 19 kDa. The expected band size for Cyclophilin B is at 24 kDa.
Product group Antibodies
Boster Bio
Anti-Cyclophilin B/PPIB Antibody Picoband(r)A03229-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPPIB
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Product group Antibodies
References
GeneTex
Cyclophilin B antibodyGTX118372
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPPIB
  • SizePrice
Product group Antibodies
Assay Genie
Cyclophilin B Monoclonal AntibodyCAB4861
TargetPPIB
  • SizePrice
Product group Antibodies
Biomatik
Anti-Cyclophilin B (CYPB) Monoclonal AntibodyCAU36248
ApplicationsFlow Cytometry
TargetPPIB
  • SizePrice
Product group Antibodies
Biosynth
Cyclophilin B antibody10R-1086
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Product group Antibodies
RayBiotech
Anti-Cyclophilin B Antibody102-25113
TargetPPIB
  • SizePrice
Product group Antibodies
Bioss Antibodies
Cyclophilin B Monoclonal AntibodyBSM-33228M
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPPIB
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb91245 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PPIB AntibodyAMAB91245
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ReactivityHuman, Mouse, Rat
TargetPPIB
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Antibodies.com
Anti-PPIB AntibodyA97954
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LifeSpan BioSciences
PPIB / Cyclophilin B Antibody (clone 4D7)LS-C769784
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TargetPPIB
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