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Western Blot analysis of COS7 cells using CYCS Polyclonal Antibody
Western Blot analysis of COS7 cells using CYCS Polyclonal Antibody
Western Blot analysis of COS7 cells using CYCS Polyclonal Antibody

CYCS Antibody

CSB-PA001922
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCYCS
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYCS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54205
  • Target name
    CYCS
  • Target description
    cytochrome c, somatic
  • Target synonyms
    CYC, HCS, THC4, cytochrome c
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP99999
  • Protein Name
    Cytochrome c
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PB9334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (PB9334) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
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