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ProductsBack/CYCS Monoclonal Antibody
Western Blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse liver, 5) Rat liver, 6) Rat kidney with CYCS Monoclonal Antibody.
Western Blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse liver, 5) Rat liver, 6) Rat kidney with CYCS Monoclonal Antibody.
Western Blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse liver, 5) Rat liver, 6) Rat kidney with CYCS Monoclonal Antibody.

CYCS Monoclonal Antibody

E-AB-22110
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CYCS Monoclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PB9334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (PB9334) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
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