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Western Blot analysis of HUVEC cells using CYP24A1 Polyclonal Antibody
Western Blot analysis of HUVEC cells using CYP24A1 Polyclonal Antibody
Western Blot analysis of HUVEC cells using CYP24A1 Polyclonal Antibody

CYP24A1 Antibody

CSB-PA007611
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP24A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP24A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1591
  • Target name
    CYP24A1
  • Target description
    cytochrome P450 family 24 subfamily A member 1
  • Target synonyms
    1,25-@dihydroxyvitamin D3 24-hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; CP24; CYP24; cytochrome P450 24A1; cytochrome P450, family 24, subfamily A, polypeptide 1; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); cytochrome P450-CC24; exo-mitochondrial protein; HCAI; HCINF1; P450-CC24; vitamin D 24-hydroxylase; vitamin D(3) 24-hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07973
  • Protein Name
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug, Lane 6: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP24A1 antigen affinity purified polyclonal antibody (Catalog # PB9547) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP24A1 at approximately 59 kDa. The expected band size for CYP24A1 is at 59 kDa.
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