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ProductsBack/CYP24A1 Polyclonal Antibody
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP24A1) polyclonal Antibody, Unconjugated (bs-3899R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP24A1) polyclonal Antibody, Unconjugated (bs-3899R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP24A1) polyclonal Antibody, Unconjugated (bs-3899R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

CYP24A1 Polyclonal Antibody

BS-3899R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYP24A1
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Overview

  • Supplier
    Bioss
  • Product Name
    CYP24A1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1591
  • Target name
    CYP24A1
  • Target description
    cytochrome P450 family 24 subfamily A member 1
  • Target synonyms
    CP24, CYP24, HCAI, HCINF1, P450-CC24, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 1,25-@dihydroxyvitamin D3 24-hydroxylase, 24-OHase, cytochrome P450 24A1, cytochrome P450, family 24, subfamily A, polypeptide 1, cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase), cytochrome P450-CC24, exo-mitochondrial protein, lncBCAS1-4_1, vitamin D 24-hydroxylase, vitamin D(3) 24-hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07973
  • Protein Name
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: A431 Whole Cell Lysate at 40ug, Lane 6: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP24A1 antigen affinity purified polyclonal antibody (Catalog # PB9547) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP24A1 at approximately 59 kDa. The expected band size for CYP24A1 is at 59 kDa.
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