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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA10349A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA10349A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA10349A0Rb at dilution of 1:100

CYR61 Antibody

CSB-PA10349A0RB
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCCN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYR61 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3491
  • Target name
    CCN1
  • Target description
    cellular communication network factor 1
  • Target synonyms
    CCN family member 1; CYR61; cysteine rich angiogenic inducer 61; cysteine-rich heparin-binding protein 61; cysteine-rich, anigogenic inducer, 61; GIG1; IBP-10; IGF-binding protein 10; IGFBP10; IGFBP-10; insulin-like growth factor-binding protein 10; protein CYR61
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00622
  • Protein Name
    Protein CYR61
  • Scientific Description
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCN1 using anti-CCN1 antibody (PB9549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: A431 Whole Cell Lysate at 40ug, Lane 4: SGC Whole Cell Lysate at 40ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1 antigen affinity purified polyclonal antibody (Catalog # PB9549) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCN1 at approximately 42 kDa. The expected band size for CCN1 is at 42 kDa.
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