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ProductsBack/CCN1 Polyclonal Antibody
U251 lysates probed with Cry61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
U251 lysates probed with Cry61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
U251 lysates probed with Cry61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

CCN1 Polyclonal Antibody

BS-1290R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCN1
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyr61 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3491
  • Target name
    CCN1
  • Target description
    cellular communication network factor 1
  • Target synonyms
    CYR61, GIG1, IGFBP10, CCN family member 1, IBP-10, IGF-binding protein 10, IGFBP-10, cysteine rich angiogenic inducer 61, cysteine-rich heparin-binding protein 61, cysteine-rich, anigogenic inducer, 61, insulin-like growth factor-binding protein 10, protein CYR61
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00622
  • Protein Name
    Protein CYR61
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCN1 using anti-CCN1 antibody (PB9549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: A431 Whole Cell Lysate at 40ug, Lane 4: SGC Whole Cell Lysate at 40ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1 antigen affinity purified polyclonal antibody (Catalog # PB9549) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCN1 at approximately 42 kDa. The expected band size for CCN1 is at 42 kDa.
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