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ProductsBack/Cystatin C (CST3) Mouse Monoclonal Antibody [Clone ID: Cyst10]

Cystatin C (CST3) Mouse Monoclonal Antibody [Clone ID: Cyst10]

AM05633PU-N
OriGene
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetCST3
Price on request
200 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cystatin C (CST3) Mouse Monoclonal Antibody [Clone ID: Cyst10]
  • Delivery Days Customer
    14
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Cyst10
  • Gene ID1471
  • Target name
    CST3
  • Target description
    cystatin C
  • Target synonyms
    ADLDWA, ARMD11, HEL-S-2, cystatin-C, bA218C14.4 (cystatin C), cystatin 3, epididymis secretory protein Li 2, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin
  • Host
    Mouse
  • Isotype
    IgG3
  • Protein IDP01034
  • Protein Name
    Cystatin-C
  • Scientific Description
    Cystatin C (CST3) mouse monoclonal antibody, clone Cyst10, Purified
  • Reactivity
    Human
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
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