
Immunohistochemical analysis of paraffin-embedded hepatoma, using Cytokeratin 18 antibody(GTX112977) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Cytokeratin 18 antibody [N1N3]
GTX112977
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKRT18
Overview
- SupplierGeneTex
- Product NameCytokeratin 18 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.83 mg/ml
- ConjugateUnconjugated
- Gene ID3875
- Target nameKRT18
- Target descriptionkeratin 18
- Target synonymsCK-18, CYK18, K18, keratin, type I cytoskeletal 18, cell proliferation-inducing gene 46 protein, cytokeratin 18, keratin 18, type I
- HostRabbit
- IsotypeIgG
- Protein IDP05783
- Protein NameKeratin, type I cytoskeletal 18
- Scientific DescriptionKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Salina ACG, Dos-Santos D, Rodrigues TS, et al. Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells. Elife. 2022,11. doi: 10.7554/eLife.74443Read this paper
- Huang HJ, Lin CC, Chou HC, et al. Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells. Mol Biosyst. 2014,10(12):3086-100. doi: 10.1039/c4mb00451eRead this paper
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000. Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112977/GTX112977_40408_20161006_WB_shRNA_watermark_w_23060500_640.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112977/GTX112977_40408_20161117_WB_2_w_23060500_945.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112977/GTX112977_40408_20161117_WB_w_23060500_647.webp)



![FACS analysis of HeLa cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R]. Blue : Primary antibody Red : Isotype control](https://www.genetex.com/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_FACS_w_23053122_467.webp)
![IHC-P analysis of human colon carcinoma section using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02658/GTX02658_20210319_IHC-P_w_23053122_304.webp)
![IHC-P analysis of human gastric antrum tissue using GTX04374 Cytokeratin 18 antibody [MSVA-118R] HistoMAX?. Strong cytokeratin 18 staining of all epithelial cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04374/GTX04374_20230728_IHC-P_39_23072722_592.webp)


![IHC-P analysis of human colon tissue using GTX57172 Cytokeratin 18 antibody [IHC018] (10X)](https://www.genetex.com/upload/website/prouct_img/normal/GTX57172/GTX57172_20180619_IHC-P_w_23061123_313.webp)