Bio-Connect
Cytokeratin 8/18(C-51), Biotin conjugate, 0.1mg/mL [26628-22-8]

Cytokeratin 8/18(C-51), Biotin conjugate, 0.1mg/mL [26628-22-8]

BNCB0034
Biotium
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biotium
  • Product Name
    Cytokeratin 8/18(C-51), Biotin conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C-51
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID3856
  • Target name
    KRT8
  • Target description
    keratin 8
  • Target synonyms
    CARD2; CK8; CK-8; CYK8; cytokeratin-8; K2C8; K8; keratin 8, type II; keratin, type II cytoskeletal 8; KO; type-II keratin Kb8
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Formalin-fixed and rat colon tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT8
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT8
  • SizePrice
Product group Antibodies
Assay Genie
TargetKRT8
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
TargetKRT8
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ELISA
  • SizePrice
IHC staining of human colon cancer tissue, diluted at 1:200.
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT8
  • SizePrice
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetKRT8
  • SizePrice
Product group Antibodies
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetKRT8
  • SizePrice
Figure 1. Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (A01421). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetKRT8
  • SizePrice