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Cytokines Growth Factors

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Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Product group Proteins / Signaling Molecules
GeneTex
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Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
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Deglycosylation of GTX65642-pro Progranulin (mouse) protein.To examine the deglycosylation of mouse Progranulin, 1 μg of mouse progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (mouse) antibody.
Product group Proteins / Signaling Molecules
GeneTex
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
Protein IDP10749
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Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice

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