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DAP Kinase 1 antibody [DAPK-55]

GTX10443
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetDAPK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DAP Kinase 1 antibody [DAPK-55]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DAPK-55
  • Conjugate
    Unconjugated
  • Gene ID1612
  • Target name
    DAPK1
  • Target description
    death associated protein kinase 1
  • Target synonyms
    DAPK, ROCO3, death-associated protein kinase 1, DAP kinase 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP53355
  • Protein Name
    Death-associated protein kinase 1
  • Scientific Description
    DAP Kinase 1 (death associated protein kinase) is a calmodulin dependent serine/threonine kinase that carries ankyrin repeats and the death domain. DAPK transfectants showed increased sensitivity in vitro to apoptotic stimuli. DAP Kinase 1 may function as a tumor suppressor gene.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Franchi M, Karamanos KA, Cappadone C, et al. Colorectal Cancer Cell Invasion and Functional Properties Depend on Peri-Tumoral Extracellular Matrix. Biomedicines. 2023,11(7). doi: 10.3390/biomedicines11071788
    Read this paper
  • Franchi M, Karamanos KA, Cappadone C, et al. Substrate Type and Concentration Differently Affect Colon Cancer Cells Ultrastructural Morphology, EMT Markers, and Matrix Degrading Enzymes. Biomolecules. 2022,12(12). doi: 10.3390/biom12121786
    Read this paper
  • Masola V, Bonomini M, Onisto M, et al. Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis. Nutrients. 2021,13(7). doi: 10.3390/nu13072282
    Read this paper

Datasheet

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Figure 1. Western blot analysis of DAPK1 using anti-DAPK1 antibody (A01161-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAPK1 antigen affinity purified polyclonal antibody (Catalog # A01161-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAPK1 at approximately 160 kDa. The expected band size for DAPK1 is at 160 kDa.
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