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WB analysis of various sample lysates using GTX54047 DAP Kinase 1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54047 DAP Kinase 1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54047 DAP Kinase 1 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

DAP Kinase 1 antibody

GTX54047
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetDAPK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DAP Kinase 1 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1612
  • Target name
    DAPK1
  • Target description
    death associated protein kinase 1
  • Target synonyms
    DAP kinase 1; DAPK; death-associated protein kinase 1; ROCO3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53355
  • Protein Name
    Death-associated protein kinase 1
  • Scientific Description
    Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAPK1 using anti-DAPK1 antibody (A01161-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAPK1 antigen affinity purified polyclonal antibody (Catalog # A01161-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAPK1 at approximately 160 kDa. The expected band size for DAPK1 is at 160 kDa.
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