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ProductsBack/DDX58 / RIG-1 / RIG-I Antibody

DDX58 / RIG-1 / RIG-I Antibody

LS-C831077
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRIGI
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DDX58 / RIG-1 / RIG-I Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:10000), IHC (1:30 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23586
  • Target name
    RIGI
  • Target description
    RNA sensor RIG-I
  • Target synonyms
    DDX58, RIG-I, RIG1, RLR-1, SGMRT2, antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, DExD/H-box helicase 58, RNA helicase RIG-I, probable ATP-dependent RNA helicase DDX58, retinoic acid-inducible gene 1 protein, retinoic acid-inducible gene I protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDX58 using anti-DDX58 antibody (A00244-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody (Catalog # A00244-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 107 kDa. The expected band size for DDX58 is at 107 kDa.
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