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Goat anti-DDX58

EB08003
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDDX58
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-DDX58 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, FC, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23586
  • Target name
    DDX58
  • Target description
    DExD/H-box helicase 58
  • Target synonyms
    antiviral innate immune response receptor RIG-I; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; probable ATP-dependent RNA helicase DDX58; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; RIG1; RIGI; RIG-I; RLR-1; RNA helicase RIG-I; SGMRT2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_055129.2. Purification: Antigen affinity purified. Names and symbols: DDX58 ; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DKFZp434J1111 ; DKFZp686N19181 ; FLJ13599; RIG-I; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide ; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I ; OTTHUMP00000045225 ; RNA helicase ; RNA helicase RIG-I
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDX58 using anti-DDX58 antibody (A00244-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody (Catalog # A00244-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 107 kDa. The expected band size for DDX58 is at 107 kDa.
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